Why does DNA absorb at 280 nm?
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Works at the International Labour Organization, Lives in Geneva, Switzerland.
For proteins, the absorbance is related to the Tyrosine, Pheynylalanine, and Tryptophan rings. ... The Phenol ring of Phenylalanine actually absorbs weakly at 260 so the dominate UV-vis features of proteins are the tyrosyl and the indole which absorb at 280 nm. Conversely, the nucleobases absorb well at 260 nm.
2023-06-18 00:23:00
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Harper Allen
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For proteins, the absorbance is related to the Tyrosine, Pheynylalanine, and Tryptophan rings. ... The Phenol ring of Phenylalanine actually absorbs weakly at 260 so the dominate UV-vis features of proteins are the tyrosyl and the indole which absorb at 280 nm. Conversely, the nucleobases absorb well at 260 nm.